The essential purpose of this research study is to determine the Assessing the impact of commercial fishing on the abundance and diversity of fish species in a marine protected area: A case study in the Mediterranean Sea. This research study is based on the theory analysis, and some portion is based on the numerical analysis. This research depends upon primary research data for collecting the data from the commercial fishermen and those persons who have knowledge about diverse fish species, all of them consider as research participants. For determining the research study used SPSS software and generated results. The overall result found that there is a positive and significant effect of commercial fishing on the abundance and diversity of fish species in a marine protected area. The variability of spatial structure in fish structure varies from habitat to habitat. For studying the different spatial structures, fish assemblage-based methods are used in MPA studies. The multivariate assemblage method provides an assumption about the spatial structure of marine fishes by finding the correlation between various variable parts.