The essential aim of this research study is to determine the role of gut microbiota in regulating immune response also that disease resistance in fish farmed. This research study represents that numerical analysis by using primary research data. For collecting the research data used, specific questions these questions fulfilled from new generation sequencing. This research study is also based on theoretical analysis for measuring the research using smart PLS software and running informative results, including the smart PLS Algorithm. The descriptive statistical analysis, the correlation coefficient, the R square, and the significant analysis also present the co-linearity statistical analysis between them. Overall results found that there are negative but significant links between the microbiota in regulating immune response and disease resistance in fish farmed