Decoding comparable morphologies: Pigmentation validated for identifying southern California Paralabrax larvae

Abstract

The distribution and trends in larval fish abundance are often used to assess the status and trends of marine fish populations. However, for
closely related species whose larvae are morphologically similar and whose genetic identities may be degraded by formalin preservation,
unraveling species-specific larval abundances from long-term monitoring efforts presents a challenge. We used statistical methods and the
molecular identities of 107 ethanol-preserved specimens to construct and test a taxonomic key based on pigmentation patterns observed in
three species of Paralabrax (family Serranidae) from southern California. Previously, larvae of these species were not thought to be reliably
distinguishable based on morphology or pigmentation. However, when using pigmentation characters paired with molecular identities, a
Random Forest Classifier provided a tool for structuring and refining a taxonomic key to distinguish species. Following calibration and
key refinement, the probabilities of achieving accurate and precise species classifications using our taxonomic key were >96%, indicating
that ventral and pectoral fin pigmentation patterns can discriminate Paralabrax larvae. Importantly, we can now leverage existing and
future ichthyoplankton survey collections to assess species-specific trends in larval abundance without requiring expensive and labintensive
genetic analyses used with formalin-fixed specimens.